Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.

doi: 10.4049/jimmunol.175.9.6177

Figure Lengend Snippet: FIGURE 1. IL-17R expression on human NSCLC lines. Three different human NSCLC lines, Sq-19, A549, and LK-87, were incubated with mouse anti-human IL-17R mAb (solid line) or irrelevant mouse IgG1 (dotted line), followed by PE-conjugated goat anti-mouse IgG1 Ab. The expression of IL-17R on the surface of NSCLC cells was analyzed by flow cytometry.

Article Snippet: Cells (1 106), washed twice with PBS containing 2% FCS and 0.1% NaN3, were incubated with mouse anti-human IL-17R mAb or with irrelevant normal mouse IgG1 (R&D Systems) at 4°C for 60 min. After washing, the cells were incubated with PE-conjugated goat anti-mouse IgG1 Ab (Caltag Laboratories) at 4°C for 30 min.

Techniques: Expressing, Incubation, Cytometry

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.

doi: 10.4049/jimmunol.175.9.6177

Figure Lengend Snippet: FIGURE 9. IL-17 and IL-17R expression and vascularity in human NSCLC tissues. A, Total cellular RNA was extracted from human NSCLC tissues. Five micrograms of total RNA were applied for the synthesis of cDNAs. IL-17 mRNA expression was detected in five of seven NSCLC

Article Snippet: Cells (1 106), washed twice with PBS containing 2% FCS and 0.1% NaN3, were incubated with mouse anti-human IL-17R mAb or with irrelevant normal mouse IgG1 (R&D Systems) at 4°C for 60 min. After washing, the cells were incubated with PE-conjugated goat anti-mouse IgG1 Ab (Caltag Laboratories) at 4°C for 30 min.

Techniques: Expressing

Journal: Cell reports

Article Title: IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury

doi: 10.1016/j.celrep.2022.111848

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used: mouse anti-NF200 (1:200, Sigma), rabbit anti-MBP (1:500, Calbiochem), goat anti-PDGFRα (1:500; Neuromics), mouse anti-HA (1:1000, Biolegend), rabbit-Gst-π (1:1000, Millipore), rabbit anti-IL-17Rb (1:500, Santa Cruz Biotech), rat anti-CXCL5 (1:250, R&D) in PBS containing 5% goat or donkey serum and 0.3% Triton-X 100 (Sigma) overnight at 4°C.

Techniques: Blocking Assay, Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Luminex, Hybridization, Plasmid Preparation, Biomarker Discovery, Control, Software